Circuit builder

iGEM Thessaly Circuit builder

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Parts

Click or drag onto the DNA line

Red

Green

Blue

TF1

TF2

TF3

TF4

Promoter (cAMP)

Promoter (TF1 OR TF2)

Promoter (TF4 AND TF3)

Constitutive

Promoter (TF1)

Promoter (TF2)

Promoter (TF3)

Promoter (TF4)

Inhibitor

Operator

Terminator

Red

Produces red fluorescent protein.

Green

Produces green fluorescent protein.

Blue

Produces blue fluorescent protein.

TF1

Produces TF1.

TF2

Produces TF2.

TF3

Produces TF3.

TF4

Produces TF4.

Promoter (cAMP)

Inducible promoter — activated by cAMP. Initiates transcription of downstream genes.

Promoter (TF1 OR TF2)

Inducible promoter — activated by TF1 or TF2.

Promoter (TF4 AND TF3)

Inducible promoter — activated by TF4 and TF3.

Constitutive

Always-on promoter. Initiates transcription without any ligand.

Promoter (TF1)

Promoter activated by TF1 ligand.

Promoter (TF2)

Promoter activated by TF2 ligand.

Promoter (TF3)

Promoter activated by TF3 ligand.

Promoter (TF4)

Promoter activated by TF4 ligand.

Promoter (All)

Promoter activated by all ligands.

Inhibitor

Produces an inhibitor protein. Blocks the lac Operator.

Operator

Controls transcription flow.

Terminator

Stops transcription at this point.

Ligands

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